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Phosphorylated and unphosphorylated forms of human single-stranded DNA-binding protein are equally active in simian virus 40 DNA replication and in nucleotide excision repair.

机译：人单链DNA结合蛋白的磷酸化和非磷酸化形式在猿猴病毒40 DNA复制和核苷酸切除修复中具有同等活性。

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The trimeric human single-stranded DNA-binding protein (HSSB; also called RP-A) plays an essential role in DNA replication, nucleotide excision repair, and homologous DNA recombination. The p34 subunit of HSSB is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation. We have previously shown that the phosphorylation of p34 is catalyzed by both cyclin-dependent kinase-cyclin A complex and DNA-dependent protein kinase. In this study, we investigated the effect of phosphorylation of p34 by these kinases on the replication and repair function of HSSB. We observed no significant difference with the unphosphorylated and phosphorylated forms of HSSB in the simian virus 40 DNA replication or nucleotide excision repair systems reconstituted with purified proteins. The phosphorylation status of the p34 subunit of HSSB was unchanged during the reactions. We suggest that the phosphorylated HSSB has no direct effect on the basic mechanism of DNA replication and nucleotide excision repair reactions in vitro, although we cannot exclude a role of p34 phosphorylation in modulating HSSB function in vivo through a yet poorly understood control pathway in the cellular response to DNA damage and replication.

机译：三聚体人单链DNA结合蛋白（HSSB；也称为RP-A）在DNA复制，核苷酸切除修复和同源DNA重组中起重要作用。 HSSB的p34亚基在细胞周期的G1 / S边界处或细胞暴露于DNA损伤诱导剂（包括电离和UV辐射）时被磷酸化。以前我们已经表明，细胞周期蛋白依赖性激酶-细胞周期蛋白A复合物和DNA依赖性蛋白激酶都可以催化p34的磷酸化。在这项研究中，我们研究了这些激酶对p34的磷酸化对HSSB复制和修复功能的影响。我们观察到猿猴病毒40 DNA复制或用纯化蛋白重建的核苷酸切除修复系统中，HSSB的未磷酸化和磷酸化形式没有显着差异。在反应过程中，HSSB的p34亚基的磷酸化状态没有变化。我们建议磷酸化的HSSB对体外DNA复制和核苷酸切除修复反应的基本机制没有直接影响，尽管我们不能排除p34磷酸化通过尚不清楚的细胞内控制途径在体内调节HSSB功能中的作用。对DNA损伤和复制的反应。

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Pan, Z Q; Park, C H; Amin, A A; Hurwitz, J; Sancar, A;
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年度 1995
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1. Phosphorylated and unphosphorylated forms of human single-stranded DNA-binding protein are equally active in simian virus 40 DNA replication and in nucleotide excision repair. [J] . Pan ZQ, Park CH, Amin AA, Proceedings of the National Academy of Sciences of the United States of America . 1995,第10期

机译：人单链DNA结合蛋白的磷酸化和非磷酸化形式在猿猴病毒40 DNA复制和核苷酸切除修复中具有同等活性。
2. Identification of the Simian Virus 40 Which Replicates When Simian Virus 40-Transformed Human Cells Are Fused with Simian Virus 40-Transformed Mouse Cells or Superinfected with Simian Virus 40 Deoxyribonucleic Acid [J] . Saul Kit, T. Kurimura, McKay Brown, Journal of Virology . 1970,第1期

机译：鉴定Simian病毒40，当Simian病毒40转化的人体细胞与Simian病毒40转化的小鼠细胞融合或用Simian病毒40脱氧核糖核酸进行超育
3. Identification of the Simian Virus 40 Which Replicates When Simian Virus 40-Transformed Human Cells Are Fused with Simian Virus 40-Transformed Mouse Cells or Superinfected with Simian Virus 40 Deoxyribonucleic Acid [J] . Saul Kit, T. Kurimura, McKay Brown, Journal of Virology . 1970,第1期

机译：鉴定Simian病毒40，当Simian病毒40转化的人体细胞与Simian病毒40转化的小鼠细胞融合或用Simian病毒40脱氧核糖核酸进行超育
4. Nucleotide Fluctuation of Radiation-Resistant Halobacterium sp. NRC-1 Single-Stranded DNA-Binding Protein (RPA) Genes [C] . Todd Holden, G. Tremberger Jr., E. Cheung, Instruments and methods for astrobiology and planetary missions XII . 2009

机译：耐辐射盐杆菌的核苷酸波动NRC-1单链DNA结合蛋白（RPA）基因
5. Roles of the mitochondrinal single-stranded DNA-binding protein at the mitochondrial DNA replication fork [D] . Oliveira, Marcos Tulio 2011

机译：线粒体单链DNA结合蛋白在线粒体DNA复制叉中的作用
6. Phosphorylated and unphosphorylated forms of human single-stranded DNA-binding protein are equally active in simian virus 40 DNA replication and in nucleotide excision repair. [O] . Z Q Pan, C H Park, A A Amin, 1995

机译：人单链DNA结合蛋白的磷酸化和非磷酸化形式在猿猴病毒40 DNA复制和核苷酸切除修复中具有同等活性。
7. A role for the human single-stranded DNA binding protein HSSB/RPA in an early stage of nucleotide excision repair. [O] . Coverley, D, Kenny, M K, Lane, D P, 1992

机译：人类单链DNA结合蛋白HSSB / RPA在核苷酸切除修复的早期阶段中的作用。
8. SV40 large tumor antigen and the tumor suppressor p53 are phosphorylated by a DNA-activated protein kinase from human cells. [R] . Lees-Miller, S. P., Chen, Y. R., Anderson, C. W. 1990

机译：sV40大肿瘤抗原和肿瘤抑制因子p53被来自人细胞的DNa活化蛋白激酶磷酸化。

Phosphorylated and unphosphorylated forms of human single-stranded DNA-binding protein are equally active in simian virus 40 DNA replication and in nucleotide excision repair.

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